Create a 2- to 3-page document in Microsoft Word for providing answers to s in the following: Exercise4: Purebacterialcolonies 1. Whenanagarplateis inoculatedwhyis theloopsterilizedaftertheinitialinoculumis puton? 2. Distinguishbetweena purecultureandamixedculture. 3. Definea bacterialcolony.Listfourcharacteristicsbywhichbacterialcoloniesmaybe distinguished. 4. Whyshoulda Petridishnotbe leftopenforanyextendedperiod? 5. Whydoesthestreakingmethod youusedto inoculateyourplatesresultin isolatedcolonies? Exercise5: Pourplateandstreakingtechniquetoobtainpurecultures 1. Discusstherelativeconvenienceofpour-andstreak-platetechniquesinculturingclinical specimens. 2. Howdoyoudecidewhichcoloniesshouldbepickedfrom aplatecultureofamixedflora? 3. Whyisitnecessarytomakepuresubculturesoforganismsgrownfrom clinicalspecimens? 4. Whatkindsofclinicalspecimensmayyielda mixedflorainbacterialcultures? 5. Whenmorethanonecolonytypeappearsinpureculturewhatarethemostlikelysourcesof extraneouscontamination? Exercise3: Primarymediaforisolationofmicroorganisms 1.Definea differentialmediumanddiscussitspurpose. 2.Definea selectivemediumanddescribeitsuses. 3.WhyisMacConkeyagarselectiveas wellasdifferential? 4.Whyisbloodagarusefulasaprimaryisolationmedium? 5.Whatis themajordifferencebetweenModifiedThayer-Martin(MTM)andchocolateagar?Whenwould youuseMTMratherthanchocolateagar? Support your responses with examples. Cite any sources in APA format
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