Your friend just started a work-study position in a biology lab. They are not really confident in their skills and are worried about doing something wrong. Youâve been trying to share what youâve learned about biology. Youâve received a call from your friend. Theyâve been asked to perform a PCR, theyâve designed primers, extracted the DNA, and are all ready to go but they seem to have misplaced their protocol (instructions). They need your help!What reagents should they add to their PCR reaction and what does each reagent do? Note: Donât worry about quantities, just list what goes into a PCR reaction. Explanations for function donât have to be more than one or two sentences each. Revisit your virtual lab if youâre unsure.
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