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successful intra-cytoplasmic sperm injection

Discuss all the steps leading to a successful intra-cytoplasmic sperm
injection (ICSI) including egg preparation, setting up of equipment and
tools, preparation of sperm and steps to deliver sperm through oolemma,
and discuss for each stage what developments have been made and what
can potentially affect the outcome of the ICSI procedure using published
scientific literature.

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