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Assignment -2

Answer THREE questions Ql. Answer ALL parts of this question

(a) Suggest a typical 2D NMR experiment that can be used for the elucidation of drug protein interactions. Critically appraise the fundamentals of this technique and with the aid of an example, highlight how results can be monitored.
[18 marks]

(b) You have been provided with a sample that contains a counterfeit mixture of drugs. Critically describe, with the aid of a figure, an NMR experiment that can be used to separate and identify the components in this mixture. [15 marks] [Total 33 marks]

Q3. Answer ALL parts of this question The following questions are related to chiral separations:

(a) Discuss why pharmaceutical companies care about the chiral purity of their compounds. [8 marks]

(b) Discuss how racemic mixtures are separated. [15 marks]

(c) If you must validate a specific chromatography methodology, discuss how you evaluate the key validation parameters. [10 marks] [Total 33 marks]

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